Dr. N.D.Pradeep Singh's

Research Page

 

 

Design and development of fluorescent photoremovable protecting groups for controlled drug delivery.

 

Photoremovable protecting groups are of great interest since they allow precise spatial and temporal control over the release of the protected functionalities. Our group has designed and synthesized various photoactivable protecting groups which can cage and release bioactive molecules, with substitutions like alcohols, acids, amines etc., at specific site, upon exposure to UV or visible light independent of the reaction media. Further by tuning the substituents we made it possible to control the wavelength and rate of release. Photocaging refers to temporary inactivation of a biologically active molecule using a photoremovable protecting group (PRPG). Our group further utilized these photoremovable protecting groups for the controlled release of drugs and real time monitoring of the drug released. In this process, since the drug molecule is covalently attached with a photoactive molecule, it loses its activity temporarily, thereby ruling out the possibility of premature release or leaching of the drug. Once the system reaches the target site, the drug can be released from the photoactive molecule through photolysis, thus restoring its activity. Some of the newly developed protecting groups by our group are perylen-3-ylmethyl, 1-acetylpyrene, 1-hydroxyacetylpyrene, acridine-9-ylmethyl, acetyl carbazole. These newly developed protecting groups are highly fluorescent and can absorb in the visible region. He has utilized these protecting groups in releasing single and dual anticancer drugs. Our group has recently developed p-hydroxyphenacyl-benzothiazole-chlorambucil conjugate as a real time monitoring drug delivery system assisted by excited state intramolecular proton transfer. Here, we have modified a well-known phototrigger, the p-hydroxyphenacyl (pHP) group by making it fluorescent and increasing its excitation wavelength above 400 nm so that it can be efficiently utilized as drug delivery agent in the area of theranostics.

 

 

 

 

Photoremovable Protecting Groups as Organic Nanocarriers for Image Guided Drug Delivery

Our group has developed nanoparticle-sized photoremovable protecting group for the first time, where PRPGs by itself can act as a nanocarrier for the drug delivery, thereby eliminating the key steps involved in synthesizing dual component photoresponsive nanocarriers. Our group has developed drug conjugated Perylene-3-ylmethanol, Acridin-9-methanol, 1-Acetylpyrene fluorescent photocages as organic nanoparticles. Perylene-3-ylmethanol organic nanoparticles showed real-time monitoring of anticancer drug release. In vitro biological studies of all these nanoparticles revealed that these newly synthesized organic nanoparticles exhibit good biocompatibility and cellular uptake as well as efficient photoregulated anticancer drug release ability. Such fluorescent organic nanoparticles which were developed by our group may open up new perspectives for designing a new class of promising photoresponsive nanocarriers for drug delivery.


Photoresponsive Inorganic Nanocarriers for Image Guided Drug Delivery

Generally, photoresponsive nanoparticles for drug delivery are constructed using two main ingredients: biocompatible nanoparticles and photoremovable protecting groups, which are nothing but small organic molecules which are able to release active molecule in presence of light. To date our group has developed various photoresponsive nanoparticles such as coumarin tethered Fe/Si magnetic nanoparticles, quinoline decorated mesoporous silica nanoparticles, quinoline bounded carbon dots and silicon quantum dots for image guided drug delivery in vitro. With these newly developed functionalized organic and inorganic nanoparticles our group has shown efficient cell targeted drug delivery, nuclear targeted drug delivery via charge reversal and real time monitoring of the drug release via in vitro experiments.

 

 

 

Sensing and Photoresponsive Drug Delivery

Cancer thrives in an acidic environment, and doesn't survive in an normal, more alkaline environment. Cancer cells make our body even more acidic as they produce lactic acid. Singh's group has utilized this tumor microenvironment to develop pH sensing and drug delivery systems. We developed an ESIPT based drug delivery system for pH sensitive fluorescence properties and photocontrolled release of the anticancer drug chlorambucil.

According to past research, healthy human prostate accumulates the highest level of zinc of any soft tissue in the body. But in the contrary, with the development of malignancy, there is a dramatic reduction in the zinc content of prostate tissue, associated with the inability of cancer cells to accumulate zinc. Our group has developed system that first guided us to locate the diseased area by using cellular zinc concentration as a biomarker followed by on-demand release of anticancer drug chlorambucil by employing the external stimulus, light.

Biological thiols, including glutathione(GSH), cysteine (Cys), and homocysteine (Hcy), play crucial roles in maintaining the appropriate redox status of biological systems. GSH is the most abundant cellular thiol, which is important for maintaining cellular defense against free radicals and reactive oxygen species (ROS), and it also maintains exogenous antioxidants in their reduced forms. Our group has designed a redox-responsive drug delivery system (DDS) to exploit the higher level of thiols in tumor sites to trigger the release of drug from the DDS.