Electron Density in the Multiscale Treatment of Biomolecules
Authors: S. Karmakar, S. Muduli, A. Paul, and S. MishraJournal: In Fundamentals of Electron Density. Nova Science Publishers, Year: 2023 Posted: 03 Jan 2023
Authors: S. Palei, J. Jose, H. D. MootzVol: 2371 Pages: 193-213Journal: Methods in Molecular Biology Year: 2022 Posted: 12 Aug 2024
Semisynthetic cyclic peptides bearing both non-proteinogenic and genetically encoded amino acids are excellent ligands for peptide-based drug discovery. While semisynthesis expands the chemical space, genetic encoding allows access to a large library via randomization at the nucleic acid level. Selection of novel binders of such macrocyclic ligands requires linking their genotype to phenotype. In this chapter, we report a bacterial cell-surface display system to present cyclic peptides composed of synthetic and genetically encoded fragments. The synthetic fragment along with the split intein partner and an aminooxy moiety is ligated and cyclized with the recombinant backbone containing an unnatural amino acid by protein trans-splicing and intramolecular oxime ligation, respectively. A pH-shift protocol was applied to accelerate on surface cyclization. This method will enable generation of semisynthetic cyclic peptide libraries and their selection by fluorescence-activated cell sorting.<\/p>")">
A review on recent application of proton transfer photophysics of bipyridine-3,3?-diol in organized assemblies
Authors: Journal: Journal of Chemical Sciences Year: 2022 Posted: 07 Jul 2023
State of the Art and Perspectives on the Biofunctionalization of Fluorescent Metal Nanoclusters and Carbon Quantum Dots for Targeted Imaging and Drug Delivery
Authors: Sangita Kundu, Meghna Ghosh, and Nilmoni SarkarVol: 37, 31 Pages: 9281–9301Journal: Langmuir Year: 2021 Posted: 23 Aug 2021
Dynamics of the vesicles composed of fatty acids and other amphiphile mixtures: unveiling the role of fatty acids as a model protocell membrane
Authors: Niloy Kundu, Dipankar Mondal & Nilmoni SarkarJournal: Biophysical Reviews Year: 2020 Posted: 07 Jul 2023
Nanomaterials for Electrochemical Energy Storage Devices
Authors: Poulomi Roy and Suneel Kumar SrivastavaJournal: Scrivener-Wiley Publishing, USA Year: 2019 Posted: 02 Dec 2021
Surfactant Behavior of Ionic Liquids Involving a Drug
Authors: A. Pyne, S. Kundu, N. Sarkar.Journal: Springer Nature Singapore Pte Ltd. 2019 S. Zhang (ed.), Encyclopedia of Ionic Liquids, https://doi.org/10.1007/978-981-10-6739-6_5-1 Year: 2019 Posted: 05 Apr 2019
Investigating the Dynamics and Organization of Membrane Proteins and Lipids by Imaging Fluorescence Correlation Spectroscopy
Authors: N. Bag, S. Huang, T. WohlandJournal: Springer Biophysics Series: Membrane Organization and Dynamics Year: 2018 Posted: 04 Mar 2022
Authors: S. Palei, H. D. MootzVol: 1495 Pages: 77-92Journal: Methods in Molecular Biology Year: 2017 Posted: 12 Aug 2024
Cyclic peptides are highly desired molecules not only for basic research but also for many biomedical and pharmacological applications. Due to their potentially superior physicochemical properties as compared to their linear counterparts, they are considered as ideal candidates for studying protein-protein interactions, among others. Most of the methods developed in recent years to prepare cyclic peptides focus either on a synthetic or a recombinant route. While the former provides access to diversified, noncanonical peptides, including unnatural and D-amino acid, for example, the latter can harness the power of genetic randomization to generate and select from large peptide libraries. Only few approaches have been reported to prepare semisynthetic macrocycles that would benefit from both the advantages associated with synthetic and genetically encoded parts. We describe in this chapter a chemo-enzymatic method to make semisynthetic cyclic peptides in vitro from two fragments using protein trans-splicing and bioorthogonal oxime ligation.<\/p>")">
Hybrid Nanomaterials: Developments in Energy, Environmentsand Polymer Nanocomposites
Authors: Suneel Kumar Srivastava and Vikas MittalJournal: Scrivener-Wiley Publishing, USA Year: 2017 Posted: 02 Dec 2021
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